Or pathogenicity islands) can simultaneously identify species and provide some insight into the organism’s population structure.įor patients infected with Mycobacteria, especially those coinfected with the human immunodeficiency virus type 1 and typeĢ (HIV-1, HIV-2), the identity of the Mycobacterium species and the presence of mutations that confer both biologically and clinically important phenotypes are of critical importance.īoth of these issues have implications for the appropriate care and treatment of the infected patient. This demonstrates the general point that DNA microarrays that sequence important genomic regions (such as drug resistance tuberculosis that confers rifampin resistance, this work demonstrates that this array can identify the species of nontuberculous Mycobacteria. Intended utility for sequencing the region of M. However, for several species, the number of alleles in the 16S and rpoB gene sequences provided discordant estimates of the genetic diversity within a species. Whether 16S sequence, rpoBsequence, or the pattern of rpoB hybridization was used. Species identification for each of the isolates was similar irrespective of The nucleotide sequence diversity in 121 Mycobacterial isolates (comprised of 10 species) was examined by both conventionalĭideoxynucleotide sequencing of the rpoB and 16S genes and by analysis of the rpoB oligonucleotide array hybridization patterns. High-density oligonucleotide arrays can be used to rapidly examine large amounts of DNA sequence in a high throughput manner.Īn array designed to determine the specific nucleotide sequence of 705 bp of the rpoB gene of Mycobacterium tuberculosis accurately detected rifampin resistance associated with mutations of 44 clinical isolates of M.
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